Search results for "Enzyme histochemistry"
showing 3 items of 3 documents
Congenital myopathies - a comprehensive update of recent advancements
2009
The congenital myopathies are relatively newly discovered compared with other categories of muscle diseases. Current research continues to clarify and classify the congenital myopathies. These pose a diagnostic problem and cannot be diagnosed by routine hematoxylin and eosin stain. A lot of special techniques are required to diagnose them correctly and it's various subtypes. The disease specific structural changes seen in the muscle are detected by enzyme histochemistry, immunohistochemistry and electron microscopy. Through this review we provide an up-to-date analysis of congenital myopathies including clinical and pathologic aspects.
Muscle Biopsy; Processing and Evaluation
2014
Muscle biopsy is a diagnostic procedure to recognize myopathological lesions in skeletal muscle. It may be an open surgical procedure or performed by needle biopsy. The muscle biopsy ought to target a myopathologically affected muscle, chosen by clinical features and/or myoimaging. Preparative techniques entail histology, electron microscopy, enzyme histochemistry, and immunohistochemistry, but it is essential to leave additional tissue for possible subsequent biochemical and molecular investigations. The backbone of light microscopic, diagnostic studies is unfixed frozen muscle tissue with cross-sectioned muscle fibers. Correct archiving of biopsied muscle tissue for future diagnostic and …
Congenital Myopathies in the New Millennium
2005
Few medical disciplines have benefited so enormously from the molecular revolution as myology. Whereas the congenital myopathies have flourished from enzyme histochemistry and electron microscopy, defining individual congenital myopathies by structural abnormalities, genetic research has only recently focused on congenital myopathies. However, a number of congenital myopathies have been molecularly elucidated: central and multiminicore diseases, nemaline myopathy, myotubular myopathy, and congenital myopathy marked by aggregation of proteins, giving rise to the concept of protein aggregate myopathies, to which now desminopathies, α-B crystallinopathies, selenoproteinopathy, myotilinopathy,…